Journal: Science Advances

Article Title: Dysregulation of ectonucleotidase-mediated extracellular adenosine during postmenopausal bone loss

doi: 10.1126/sciadv.aax1387

Figure Lengend Snippet: ( A to L ) Administration of BAY 60-6583 and vehicle for 8 weeks in OVX animals (4 weeks after ovariectomy). Groups are compared to healthy control with no surgery and no treatment (CTL). (A) TRAP staining (purple). Scale bar, 50 μm. (B) Quantification of TRAP-positive cells on bone surface. n = 4. (C) Double-fluorescence bone labeling by calcein (green) and alizarin complexone (red). Scale bar, 100 μm. (D) Quantification of mineral apposition rate (MAR) from bone labeling images. (E) Quantification of bone formation rate (BFR/BS) from bone labeling images. n = 5. (F) Reconstructed microCT images of L4 vertebra. Scale bar, 500 μm. (G to J) Quantification of microCT images. (G) BMD. (H) BV/TV. (I) Tb.N. (J) Tb.Sp. n = 5. Mechanical measurement for (K) maximum load and (L) stiffness of tibia. n = 12. OV, ovariectomy, vehicle [dimethyl sulfoxide (DMSO)]; OB, ovariectomy, BAY 60-6583 (1 mg/kg). * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The reconstruction of scanned images was performed using microCT Evaluation Program V6.6 (Scanco Medical), followed by generation of radiographs and three-dimensional models using microCT Ray V4.0 (Scanco Medical).

Techniques: Control, Staining, Fluorescence, Labeling

Journal: medRxiv

Article Title: A DDR2-targeted PET tracer images activated fibroblasts in early pulmonary fibrosis

doi: 10.1101/2025.11.26.25341068

Figure Lengend Snippet: a , Representative longitudinal PET/CT and CT images of unilateral bleomycin (BLM)-induced pulmonary fibrosis in hFAP-knock-in (hFAP-BLM) mice imaged with 18 F-FAPI and in wild-type C57BL/6-BLM mice imaged with 68 Ga-NOTA-1A12 at days 3, 7, 14, 21, and 28 after modeling. White dashed lines delineate the fibrotic lung regions. b , Quantitative analysis of probe uptake in fibrotic lung regions expressed as SUV mean. Data are presented as mean ± SD ( n = 8∼10 per group). Statistical significance was determined using one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns: not significant. c , Schematic diagram of the sequential dual-probe PET/CT imaging protocol in the bleomycin-induced pulmonary fibrosis crab-eating macaque model. The macaque was first imaged with 18 F-FAPI on day 4 post-BLM instillation, followed by 68 Ga-NOTA-1A12 imaging on day 6 after sufficient radioactive decay. d , Representative axial, coronal, and maximum intensity projection (MIP) PET/CT images showing lung fibrotic regions with probe accumulation.

Article Snippet: To generate stable overexpression models, lentiviral vectors carrying human DDR2 or hFAP complementary DNA (IGE Biotechnology, Guangzhou, China) were constructed and co-transfected with packaging plasmids psPAX2 and pMD2.G into 293T cells using Lipofectamine 3000 (Invitrogen).

Techniques: Positron Emission Tomography-Computed Tomography, Knock-In, Imaging

Journal: Journal of Nuclear Medicine

Article Title: Clinical Translation of a 68 Ga-Labeled Integrin α v β 6 –Targeting Cyclic Radiotracer for PET Imaging of Pancreatic Cancer

doi: 10.2967/jnumed.119.237347

Figure Lengend Snippet: (A) Chemical structure of DOTA-cycratide. (B) Inhibition of 64Cu-cycratide binding to integrin αvβ6 on BxPC-3 cells by cycratide, DOTA-cycratide, and linear-pep. Data are shown as mean ± SD, n = 4. (C) Binding of 68Ga-cycratide to BxPC-3 with or without blocking of cold cycratide or linear-pep. %AD/106 cells = percentage of total added dose per million cells. Data are shown as mean ± SD, n = 4. (D) Metabolic stability of 68Ga-cycratide in blood and urine of BALB/c mice (data are representative of 3 independent experiments). **P < 0.01.

Article Snippet: After endogenous peroxidase activity had been abolished using 0.3% hydrogen peroxide and antigen had been retrieved by microwave, tumor tissues were incubated with rabbit antihuman integrin α v β 6 antibody (bs-5791r; Bioss) overnight at 4°C.

Techniques: Inhibition, Binding Assay, Blocking Assay

Journal: Journal of Nuclear Medicine

Article Title: Clinical Translation of a 68 Ga-Labeled Integrin α v β 6 –Targeting Cyclic Radiotracer for PET Imaging of Pancreatic Cancer

doi: 10.2967/jnumed.119.237347

Figure Lengend Snippet: (A) PET imaging of orthotopic pancreatic cancer lesions in nude mice at 0.5 h after injection of 68Ga-cycratide without or with blocking dose of cold cycratide. Tumors are indicated by arrows. (B) Hematoxylin and eosin (H&E) staining of tumor tissues harvested from orthotopic tumor model. (C) Left, immunofluorescence staining of integrin αvβ6 from tumor tissues harvested from orthotopic tumor model. Right, negative control with secondary antibody only.

Article Snippet: After endogenous peroxidase activity had been abolished using 0.3% hydrogen peroxide and antigen had been retrieved by microwave, tumor tissues were incubated with rabbit antihuman integrin α v β 6 antibody (bs-5791r; Bioss) overnight at 4°C.

Techniques: Imaging, Injection, Blocking Assay, Staining, Immunofluorescence, Negative Control

Journal: Journal of Nuclear Medicine

Article Title: Clinical Translation of a 68 Ga-Labeled Integrin α v β 6 –Targeting Cyclic Radiotracer for PET Imaging of Pancreatic Cancer

doi: 10.2967/jnumed.119.237347

Figure Lengend Snippet: (A) PET/CT images of female patient with suspected pancreatic cancer. Images were obtained at 1 h after intravenous administration of 68Ga-cycratide or 18F-FDG. Tumors are indicated by arrows. (B) Immunohistochemical (IHC) staining for integrin αvβ6 in tumor sample from same patient as in A. (C) Contrast-enhanced CT (CECT) image and PET/CT images of male patient with pancreatic cancer 7 mo after surgery at 1 h after administration of 68Ga-cycratide or 18F-FDG, as well as CECT image of same patient 3 mo later (10 mo after surgery). Occupancy lesions in CT are indicated by arrows.

Article Snippet: After endogenous peroxidase activity had been abolished using 0.3% hydrogen peroxide and antigen had been retrieved by microwave, tumor tissues were incubated with rabbit antihuman integrin α v β 6 antibody (bs-5791r; Bioss) overnight at 4°C.

Techniques: Positron Emission Tomography-Computed Tomography, Immunohistochemical staining, Immunohistochemistry

Journal: Life Sciences

Article Title: Remdesivir: A potential game-changer or just a myth? A systematic review and meta-analysis ☆

doi: 10.1016/j.lfs.2020.118663

Figure Lengend Snippet: Qualitative analysis.

Article Snippet: Pasquini et al. [ ], retrospective observational study, Italy , N = 51 T = 25C = 26 M = 47 F = 4 Median age = 47 Inclusion criteria • Age more than 18 years • Positive RT-PCR essay • Severe respiratory failure with the need for mechanical ventilation Exclusion criteria • Mortality within the first 48 h , First dose of 200 mg IV on Day 1, plus 100 mg daily from Day 2 to Day 10 in the treatment group Concomitant therapies include hydroxychloroquine, tocilizumab and lopinavir/ritonavir , , Better survival with remdesivir using Charlson Comorbidity Index OR 3.506 (95% CI 1.768–6.954) Median follow up 52 days (46–57).

Techniques: Infection, Imaging, Polymerase Chain Reaction, Computed Tomography

Journal: Chemical & Biomedical Imaging

Article Title: Determining the Multivalent Effects of d -Peptide-Based Radiotracers

doi: 10.1021/cbmi.4c00071

Figure Lengend Snippet: Dynamic PET/CT imaging and ex vivo biodistribution analysis of [ 68 Ga]DPA, [ 68 Ga]DP2, and [ 68 Ga]DP3 in normal mice. (A) Dynamic PET/CT imaging of the three radiotracers in normal mice. Mice were injected with 100 μCi/mouse of [ 68 Ga]DPA, [ 68 Ga]DP2, or [ 68 Ga]DP3 via the tail vein, then immediately subjected to a 60-min dynamic PET/CT scan. (B) Ex vivo biodistribution studies of radiotracers in normal mice. Mice were intravenously injected with 50 μCi/mouse of each radiotracer. Blood, heart, liver, spleen, lung, kidney, stomach, intestine, pancreas, muscle, bone, and brain samples were collected at 5, 30, 60, and 120 min p.i. and analyzed with an autogamma counter to measure radioactivity. (C) Analysis of changes in uptake over time in eight major organs. Liver uptake was low overall, while kidney uptake showed a significant increase. The data are presented as means ± SD; n = 3.

Article Snippet: A 2480 Wizard autogamma counter (PerkinElmer WIZARD2 2480, MA, USA) was used to measure the radioactivity (counts per minute, CPM) for in vivo and in vitro evaluations.

Techniques: Positron Emission Tomography-Computed Tomography, Imaging, Ex Vivo, Injection, Radioactivity

Journal: Chemical & Biomedical Imaging

Article Title: Determining the Multivalent Effects of d -Peptide-Based Radiotracers

doi: 10.1021/cbmi.4c00071

Figure Lengend Snippet: Ex vivo biodistribution analysis of [ 68 Ga]DPA, [ 68 Ga]DP2, and [ 68 Ga]DP3 in tumor-bearing mice. (A) Ex vivo biodistribution studies of radiotracers in tumor-bearing mice. U87MG tumor model mice were injected with 50 μCi/mouse via the tail vein; At 5, 30, 60, and 120 min p.i., blood, heart, liver, spleen, lung, kidneys, stomach, intestines, pancreas, muscle, bone, brain, and tumors were harvested and analyzed for radioactivity distribution with a autogamma counter. (B) Tumor and major organ uptake over time. Tumor uptake was enhanced with multimerization. Uptake in the other organs showed slightly difference compared to normal mice. The data are presented as means ± SD; n = 3.

Article Snippet: A 2480 Wizard autogamma counter (PerkinElmer WIZARD2 2480, MA, USA) was used to measure the radioactivity (counts per minute, CPM) for in vivo and in vitro evaluations.

Techniques: Ex Vivo, Injection, Radioactivity